Extended Data Fig. 3: B cell correlates of IL7 expression.

(a) GOBP pathway analysis of genes anti-correlated with B cell IL7 (FDR < 0.05), x -axis demonstrates fold-change above background, y-axis z score, all points above dotted line FDR < 0.01, one-tailed hypergeometric test. (b) Percentage chains detected with mutation from germline (chain denoted in label) or clones containing IGHD (y axis) versus relative expression IL7 for that sample (x axis), P value refers to Spearman rank-test result, dotted lines reflect fitted linear model, shaded area 95% Confidence Interval. (c) Heatmap of key B cell subset specific genes from scRNA-seq data. (d) GOBP analysis of genes differentially expressed between B cell subsets, top 5 pathways per subset listed, one-tailed hypergeometric test. (e) UMAP from main Fig. 3e, depicting relative IL7 expression per cell. (f) Relative expression of IL7 (imputed) using scRNA-seq dataset across all B cells separated by subset (n = 28 samples). (g) Immunoglobulin chain usage in Naïve B cell subset according to patient status (HD - healthy donor n = 5, mel – metastatic melanoma n = 23); two-sided Wilcoxon rank-sum test. (h) Median expression of IL7 per B cell subset from scRNA-seq data dichotomised by rs16906115 demonstrating genotype acts through altering proportions of subsets as opposed to relative expression per cell (n = 23 patients); two-sided Wilcoxon rank-sum test. For boxplots in Extended Data Fig. 3f–h the central line reflects the median value, the box corresponds to 25%–75% quartiles, the upper whisker extends to largest value no further than 1.5x interquartile range, the lower whisker extends from 25% quartile to the smallest value no further than 1.5x interquartile range.