Extended Data Fig. 2: Conversion between renin activity and mass.

Different assays were used for the measurement of renin at the three sites. CUH and GSTT measured renin mass (mU/L), while SBH measured renin activity (nmol/L/hr). A renin mass level (pg/ml) was also sent off at selected (baseline and 6-9 month) visits at the SBH site to allow within-patient comparisons between renin mass and activity. A conversion of 1.67 was used to convert between renin mass measured in pg/ml to mU/L. The scatter plot above shows correlation between renin activity and mass within the SBH cohort. Pearson’s correlation r = 0.7851 (95% CI 0.7107 – 0.8422), p < 0.0001 (two-tailed test). For the study a conversion factor of 10.989 was used for conversion from renin activity (nmol/L/hr) to mass (mU/L), taken from the ARR thresholds for diagnosing PA: ARRactivity 1000, ARRmass 91 (1000/91 = 10.989). n = 136. CUH, Cambridge University Hospital; SBH, St Bartholomew’s Hospital; GSTT, Guy’s and St Thomas’ Hospital; ARR, aldosterone renin ratio; PA primary aldosteronism.