Extended Data Fig. 7: Microbiome in tumor and normal tissue.

a, Paired box-plot for microbial genera that were most significantly enriched in tumors compared to matched normal, and vice versa. Tumor and normal pairs are connected, red lines reflect pairs with an increased proportion in the tumor, while blue lines reflect a decreased proportion in the tumor compared to matched normal. Center line, box limits, and whiskers represent the median, interquartile range and 1.5x interquartile range respectively. b, Distinct alpha diversity matrices (InvSimpson, Chao1, Observed and Shannon) between tumor and normal colon tissue, P values were calculated using paired Mann–Whitney U-test (upper). Alpha diversity between ICR High, ICR Medium, and ICR Low, in normal (N) and tumor (T) tissues (lower), P values were calculated using an unpaired Mann–Whitney U-test. Center line, box limits, and whiskers represent the median, interquartile range and 1.5x interquartile range respectively. c, Violin plots of relative abundance of microbial genera stratified by ICR cluster (top two most significantly enriched in ICR High, and top three in ICR Low). Center line, box limits, and whiskers represent the median, interquartile range and 1.5x interquartile range respectively. ICR High, n = 59; ICR Medium, n = 128; ICR Low, n = 59. d, Number of genera significantly associated with OS and PFS using either the tumor or normal tissue. All P values are two-sided. n reflects the independent number of samples in all panels. Overall Survival (OS). Progression-Free Survival (PFS).