Extended Data Fig. 8: Relation between relative abundance of Fusobacterium and tumor characteristics in AC-ICAM.

Relative abundance of Fusobacterium (derived from 16 S rRNA gene sequencing) a-g, and Fusobacterium nucleatum (derived from WGS) h-m in relation with tumor characteristic and the tumor microenvironment in AC-ICAM. a, Box-plot for relative abundance of Fusobacterium in tumor samples by ICR cluster. Spearman correlation statistics and corresponding P value is indicated. b, Box-plots for relative abundance of Fusobacterium in tumor samples by anatomical location. c, Spearman correlation between the relative abundance of Fusobacterium in tumor samples as determined by 16 S rRNA gene sequencing and immune gene signatures d, Relative abundance of Fusobacterium in tumor samples by MSI status, hypermutation status, CMS (CMS1 vs the rest, unpaired t-test, P = 0.021), and by pathological stage, P values are calculated using unpaired t-test. e, Relative abundance of Fusobacterium in tumor samples by BRAF mutation status. Green box is not mutated, pink box with nonsynonymous mutation. f, Kaplan–Meier curves corresponding to patients with tumor samples with a relative abundance of Fusobacterium above the median compared to those below the median. Overall P value is calculated by log-rank test. Vertical lines indicate censor points. g, T cell enrichment score (ssGSEA using Bindea et al, T cell signature) in tumor samples with absence of Fusobacterium (negative) or presence (positive) (left). Stacked bar chart of distribution of T cell quartiles by Fusobacterium categories (negative, low, high) (right). h, Same as a, but for Fusobacterium nucleatum as determined by metagenomic analysis of WGS. i, Same as b, but for Fusobacterium nucleatum. j, Same as c, but for Fusobacterium nucleatum. k, Same as d, but for Fusobacterium nucleatum. l, Same as e, but for Fusobacterium nucleatum. m, Same as f, but for Fusobacterium nucleatum. n, Same as g, but for Fusobacterium nucleatum. All P values are two-sided; n reflects the independent number of samples in all panels. Overall Survival (OS). Progression-Free Survival (PFS). For all box-plots: Center line, box limits, and whiskers represent the median, interquartile range and 1.5x interquartile range respectively.