Extended Data Fig. 1: Characterization of T cell rich HCC lesions in response to PD-1 blockade. | Nature Medicine

Extended Data Fig. 1: Characterization of T cell rich HCC lesions in response to PD-1 blockade.

From: Intratumoral dendritic cell–CD4+ T helper cell niches enable CD8+ T cell differentiation following PD-1 blockade in hepatocellular carcinoma

Extended Data Fig. 1: Characterization of T cell rich HCC lesions in response to PD-1 blockade.

Surgically resected HCC lesions were isolated after two or more doses of PD-1 blockade and analyzed by H&E (N = 20 biologically independent samples) and single-cell RNA sequencing (scRNAseq N = 29 biologically independent samples). (A) Distribution of responders and non-responders across HCC etiologies (Hep B: Hepatitis B; Hep C: Hepatitis C; NASH: Non-alcoholic steatohepatitis; ASH: Alcoholic steatohepatitis). (B) Quantification of immune aggregate areas and numbers stratified by response and T cell infiltration pattern (Two sided T test). (C) Expression of cluster-defining genes by scRNAseq of key immune populations, showing number of UMI per cell. (D) Differences of cluster frequencies between tumor and adjacent tissue (Two-sided T test, adjusted for multiple-hypotheses, Benjamini–Hochberg correction). Dots represent individual study subjects. The box plot center line represents the median; box limits represent the interquartile range (IQR); whiskers represent the minimum and maximum observations greater and lesser than the IQR plus 1.5×IQR, respectively.

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