Extended Data Fig. 4: Metabolite levels in patient 1 cultured fibroblasts.

Metabolite levels in patient 1 fibroblasts cultured for 1-6 days in the presence of mutant IDH2 inhibitor relative to vehicle mean value from the corresponding time point (set to 100%, highlighted by a horizontal dotted line). a, 2-hydroxyglutaric acid (total levels); b, citric acid; c, oxidized/reduced glutathione ratio; d, 2-oxoglutaric acid. Two-sided t-test of inhibitor/vehicle ratios on log-transformed values (a,b,d) and on inhibitor/vehicle ratios from paired samples for the GSSG/GSH ratio (c); NS = statistically not significant (p > 0.05). Y-axis log-scaled except for the GSSG/GSH ratio. Data are presented as mean values +/− standard deviation. The data points represent the values of biological replicates from the inhibitor condition (N = 3 biological samples) relative to the ratio between the inhibitor and the control mean values (a,b,d) or to the mean of individual vehicle/control ratios from paired samples (c).