Extended Data Fig. 5: Impact of ERα agonists on intracellular lipids and lipotoxicity in HepG2 human hepatocytes. | Nature Medicine

Extended Data Fig. 5: Impact of ERα agonists on intracellular lipids and lipotoxicity in HepG2 human hepatocytes.

From: Interaction between estrogen receptor-α and PNPLA3 p.I148M variant drives fatty liver disease susceptibility in women

Extended Data Fig. 5

A) Representative images of Oil Red O (ORO) staining for visualization of intracellular neutral lipids of HepG2 treated for 48 h with E2 (1 μM), tamoxifen (10 μM) and DMSO as negative control. Scale bar, 200 μM. B) qRT-PCR analysis of FAR1 and SPTLC2 mRNA level in human HepG2 cell line treated for 24 h with E2 (1 μM), PPT (1 μM), DPN (1 μM), tamoxifen (10 μM), G-1 (1 μM) and DMSO as negative control. Data in panels B are presented as mean ± SEM (n = 3 independent experiments); one-way ANOVA followed by Bonferroni’s post hoc test.

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