Extended Data Fig. 4: HIV-1 neutralization dynamics between 2^nd and 3^rd visit.

(a) Time in months between baseline visit (gray dots), 2^nd visit (green dots) and 3^rd visit (turquoise dots). 44 individuals had blood draws at 3 visits. (b) Neutralization dynamics of individuals between 2^nd and 3^rd visit. Dots are the mean areas under the curves (AUC) of the IgG neutralization curves against the global panel pseudoviruses at each respective visit. Each pair of dots represent the neutralization dynamics of one individual. The red line shows a learned linear mixed model describing AUC changes between 2nd and 3rd visit. Light red areas indicate the 95% confidence bands. (c) Fitted linear mixed models in detail. Slope indicates the daily increase (positive values) or decrease (negative values) of neutralization activity in the respective groups. P-values indicate the significance of a comparison between the here used model of AUC changes over time and a model that ignores the time variable (R-function stats::anova). The left table shows models fitted to the mean AUC, which are used for visualization. The right table shows models fitted to the log2-transformed mean AUC, which are used for the calculation of half-life estimates. (d) Neutralization dynamics of participants between 2^nd visit and 3^rd visit. Dots are the mean areas under the neutralization curves (AUC) of the in vitro IgG neutralization curves against the global panel pseudoviruses at each respective visit. Each pair of dots represent the neutralization dynamics of one participant. Red lines show a learned linear mixed model describing AUC changes between baseline- and 2^nd visit. Light red areas indicate the 95% confidence bands. Half-lifes (t1/2) were calculated from the slope of separate mixed models fitted to the binary logarithm of the mean AUC.