Extended Data Fig. 5: Neutralization dynamics in elite neutralizer IDC561.
From: Dynamics and durability of HIV-1 neutralization are determined by viral replication
(a) Neutralization activity of IDC561 IgG against the global panel, YU2 and BG505 at four different time points. (b) Half maximal effective concentration (50% effective concentration (EC50)) of individual IDC561’s IgGs from different timepoints against the indicated HIV-1 Env antigens as determined by ELISA. Each dot represents the mean EC50 of two individual experiments. Line indicates the median EC50. (c) ELISA binding curves of (B) (d) Large pie charts show the numbers of heavy-chain sequences identified by single cell sorting. Smaller pie charts indicate the amount of total and clonal heavy-chain sequences as identified by single cell sorting with the respective HIV-1 Env bait. (e) Comparison of total B cell repertoire of IDC561 at the 1st and 2nd apheresis timepoints. Top panel shows frequency of IgG heavy chain variable region (VH)-genes. Bottom panel shows IgM VH-genes. Dots show results of replicates for which two independently sorted batches of IgG+ and IgM+ B cells, respectively, were used.
