Extended Data Fig. 7: LC-MS/MS detection of clone 4 from individual IDC561. | Nature Medicine

Extended Data Fig. 7: LC-MS/MS detection of clone 4 from individual IDC561.

From: Dynamics and durability of HIV-1 neutralization are determined by viral replication

Extended Data Fig. 7: LC-MS/MS detection of clone 4 from individual IDC561.The alternative text for this image may have been generated using AI.

(a) Representative tryptic peptides of 1–18 mAbs detected by LC-MS/MS. Human plasma was mixed with purified monoclonal 1–18 antibody at 1:10 or 1:100, mass ratio. Anti- BG505SOSIP.664 antibodies were purified by affinity chromatography and tryptic peptides derived from CDR1, CDR2, CDR3 region of 1–18 mAbs were identified by LC-MS/MS. The unique CDRH1 peptide ADDDPYTDDDTFTK was also detected at a 1:1,000 dilution with a low XIC value. (b) Unique tryptic CDRH1 and CDRH3 peptides derived from Clone 4 detected by LC-MS/MS at different timepoints.

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