Extended Data Fig. 6: Aggregate H3K27ac cfChIP signal at regulatory elements identified by ATAC-seq in tumor tissue.

Signal in cancer plasma (orange) and healthy plasma (gray) is compared at regulatory elements in the corresponding cancer type defined by ATAC-seq in TCGA tumors¹⁴. Dark lines show the mean signal across all samples in the indicated class. For comparison, signal at ‘common’ REs is shown, which include 10,000 regulatory elements with DNAse hypersensitivity across most or all cell types²⁰ (Methods). Boxplots indicate area under the curve for the aggregate H3K27ac profile for each sample. Lower, middle, and upper hinges indicate 25^th, 50^th, and 75^th percentiles; whiskers extend to 1.5 x the inter-quartile ranges (IQR). Wilcoxon test two-sided p-values are indicated for comparison of healthy vs cancer samples.