Extended Data Fig. 6: Evaluating MAG assembly approaches.
From: Gut microbiome evolution from infancy to 8 years of age
Summary statistics for (A) total high- (HQ) and medium-quality (MQ) post-DAS Tool MAGs (Putative Genomes) per stool, (B) average contigs per MAG, and (C) average N50 per MAG. HQ is defined as completeness ≥ 90%, contamination ≤ 5%, strain heterogeneity ≤ 0.5%, and MQ is defined as completeness ≥ 50%, contamination ≤ 5%. Boxes represent IQR with median line, and whiskers extend to 1.5xIQR. For each panel, six approaches were considered against 10 Putative Genomes: (1) timepoint-filtered (TF) long-read meta-assemblies, (2) timepoint-specific (TS) short-read meta-assemblies, (3) metaSPAdes merged with filtered long-read meta-assemblies, (4) metaSPAdes–nanopore merged with filtered long-read meta-assemblies, (5) unfiltered long-read meta-assemblies merged with timepoint-specific metaSPAdes, and (6) OPERA-MS.
