Extended Data Fig. 1: Boxplots of plasma p-tau217 (Lumipulse) concentrations in the different cohorts with AD pathology as grouping variable.

All p-tau217 levels differed significantly (P < 0.0001) between AD pathology-positive and AD pathology-negative participants in all cohorts, namely, Malmö (n = 337) (a), Gothenburg (n = 165) (b), Barcelona (n = 487) (c), Brescia (n = 230) (d), and in primary care (Sweden) (n = 548) (e). Horizontal dashed lines represent the single cutoff at 90% specificity established in (a) Secondary care Malmö. The dots represent individual participants. The central band of the boxplot represents the group median, the box limits correspond to the first and third quartiles, and the whiskers represent the minimum/maximum value or the 1.5 interquartile range (IQR) from the box, whichever is smaller. AD status was defined as CSF Aβ42/p-tau181 < 11.94 or positive amyloid PET visual read for those who did not undergo lumbar puncture. Group mean levels were compared with Student’s t-test. Abbreviations: AD, Alzheimer’s disease; IQR, interquartile range.