The community-driven initiative Quality Assessment and Reproducibility for Instruments & Images in Light Microscopy (QUAREP-LiMi) wants to improve reproducibility for light microscopy image data through quality control (QC) management of instruments and images. It aims for a common set of QC guidelines for hardware calibration and image acquisition, management and analysis.
This is a preview of subscription content, access via your institution
Relevant articles
Open Access articles citing this article.
-
FLASH: innovative integrated enzymatic-fluorescent labeling for automated muscle fiber typing, metabolic and morphometric analysis
Skeletal Muscle Open Access 21 November 2025
-
The Spheroid Light Microscopy Image Atlas for morphometrical analysis of three-dimensional cell cultures
Scientific Data Open Access 17 February 2025
-
Benchtop mesoSPIM: a next-generation open-source light-sheet microscope for cleared samples
Nature Communications Open Access 27 March 2024
Access options
Access Nature and 54 other Nature Portfolio journals
Get Nature+, our best-value online-access subscription
$32.99 / 30 days
cancel any time
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to the full article PDF.
USD 39.95
Prices may be subject to local taxes which are calculated during checkout
Change history
03 January 2022
A Correction to this paper has been published: https://doi.org/10.1038/s41592-021-01387-x
References
Baker, M. Nature 521, 274–276 (2015).
Bordeaux, J. et al. Biotechniques 48, 197–209 (2010).
Eckers, J. C., Swick, A. D. & Kimple, R. J. Radiat. Res. 189, 551–552 (2018).
Kniss, D. A. & Summerfield, T. L. Reprod. Sci. 21, 1015–1019 (2014).
Hutson, M. Science 359, 725–726 (2018).
Gibney, E. Nature 577, 14 (2020).
Clements, J. C. Nature 582, 300 (2020).
Franca, T.F. & Monserrat, J.M. EMBO Rep. 19, e46008 (2018).
Fanelli, D. Proc. Natl. Acad. Sci. USA 115, 2628–2631 (2018).
França, T. F. A. & Monserrat, J. M. Bioessays 41, e1800206 (2019).
MacNeil, T. et al. Biotechniques 69, 460–468 (2020).
Marx, V. Nat. Methods 11, 483–488 (2014).
Freedman, L. P. et al. Nat. Methods 12, 493–497 (2015).
Yu, M. et al. Nature 520, 307–311 (2015).
Ellenberg, J. et al. Nat. Methods 15, 849–854 (2018).
Sansone, S. A. et al. Nat. Biotechnol. 37, 358–367 (2019).
Nelson, G., Gelman, L., Faklaris, O., Nitschke, R. & Laude, A. Preprint at https://arxiv.org/abs/2011.08713 (2020).
Nelson, G. et al. Preprint at https://arxiv.org/abs/2101.09153 (2021).
Acknowledgements
C.M.B has been funded in part by grant number 2020-225398 from the Chan Zuckerberg Initiative DAF, an advised fund of Silicon Valley Community Foundation. The work of C.S.-D.-C. was supported by NIH grant # U01CA200059 and by grant #2019-198155 (5022) by the Chan Zuckerberg Initiative as part of their Imaging Scientist Program. R.N. was funded by Deutsche Forschungsgemeinschaft (German Research Foundation) grant number Ni 451/9-1-MIAP-Freiburg. We thank somersault18:24 BV (Leuven, Belgium) for help with Fig. 1 and Thao Do (Allen Institute, Seattle, WA, USA) for the design of the QUAREP-LiMi logo.
Author information
Authors and Affiliations
Contributions
U.B. and G.N. are chair and vice-chair of the QUAREP-LiMi’s White Paper working group (WG8). They led the effort to write this manuscript and the QUAREP white paper. C.M.B. wrote the first draft of the manuscript based on the QUAREP white paper and coordinated integration of comments and changes from the authors to realize the final version. All authors contributed to the editing of the manuscript. R.N. coordinates the QUAREP-LiMi initiative.
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing interests.
Rights and permissions
About this article
Cite this article
Boehm, U., Nelson, G., Brown, C.M. et al. QUAREP-LiMi: a community endeavor to advance quality assessment and reproducibility in light microscopy. Nat Methods 18, 1423–1426 (2021). https://doi.org/10.1038/s41592-021-01162-y
Published:
Version of record:
Issue date:
DOI: https://doi.org/10.1038/s41592-021-01162-y
This article is cited by
-
FLASH: innovative integrated enzymatic-fluorescent labeling for automated muscle fiber typing, metabolic and morphometric analysis
Skeletal Muscle (2025)
-
Light microscopy reporting for reproducibility
Nature Cell Biology (2025)
-
Reporting light microscopy data in our pages
Nature Structural & Molecular Biology (2025)
-
Crediting early-career researchers in peer review
Nature Methods (2025)
-
The Spheroid Light Microscopy Image Atlas for morphometrical analysis of three-dimensional cell cultures
Scientific Data (2025)
