Extended Data Fig. 6: TAGA-qtRNA and AGGG-qtRNA PRANCE.
From: Systematic molecular evolution enables robust biomolecule discovery
(a) Constructs for evolving TAGA-decoding qtRNAs. (b) Representative results for evolving TAGA-decoding qtRNAs. (c) Evolved qtRNAs exhibit increased ability to decode a TAGA quadruplet codon. Units are the percent luminescence when translating luxAB-357-TAGA in the presence of the qtRNA relative to expression of all-triplet-luxAB. (d) evolved genotypes E) Real-time absorbance and luminescence monitoring of qtRNA-encoding phage where either randomized or directed anticodons were used to evolve AGGG containing codons.
