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Phasor plots and the future of spectral and lifetime imaging

Nature Methods volume 20, pages 965–967 (2023)Cite this article

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I share my opinions on the benefits of and bottlenecks for hyperspectral and time-resolved imaging. I also discuss current and future perspectives for analyzing these types of data using the phasor approach.

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Fig. 1: Phasor plots for HSI and FLIM.
Fig. 2: Measurement of absolute concentrations and membrane dynamics.

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Acknowledgements

L.M. is indebted to David M. Jameson for critical reading and valuable suggestions. L.M. is supported by the grants 2020-225439, 2021-240122 and 2022-252604 from the Chan Zuckerberg Initiative DAF, an advised fund of the Silicon Valley Community Foundation; by FOCEM - Fondo para la Convergencia Estructural del Mercosur (COF 03/11); and by Comisión Sectorial de Investigación Científica (CSIC) I+D – 2020 grant #606.

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  1. Departamento de Fisiopatología, Facultad de Medicina, Hospital de Clínicas, Universidad de la República, Montevideo, Uruguay

    Leonel Malacrida

  2. Advanced Bioimaging Unit, Institut Pasteur of Montevideo and Universidad de la República, Montevideo, Uruguay

    Leonel Malacrida

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  1. Leonel Malacrida
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Correspondence to Leonel Malacrida.

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Malacrida, L. Phasor plots and the future of spectral and lifetime imaging. Nat Methods 20, 965–967 (2023). https://doi.org/10.1038/s41592-023-01906-y

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