Extended Data Fig. 1: Crystal structure and photophysical characterization of TME.
From: Global analysis of endogenous protein disorder in cells
a, Crystal structures of thiol reactive TME and its isomer 4-E. b, DFT calculations for TME and its thiol adduct TME-S showing a loss of the LUMO energy level from the maleimide and the photoinduced electron transfer effect upon reaction with thiol. c-d, Changes of TME-S fluorescence intensity in solvent mixture of ethylene glycol: glycerol with increase of glycerol fraction (vol%) demonstrating restriction of intramolecular motion (RIM) effect arising from viscosity increase (c) and in water: DMSO with increase of water fraction (vol%) demonstrating the RIM effect arising from aggregation (d). Concentration of TME: 10 µM; excitation wavelength: 322 nm; emission wavelength: 475 nm.
