Extended Data Fig. 2: TME fluorescence reveals protein unfolding in vitro.
From: Global analysis of endogenous protein disorder in cells
a, End-point emission spectra of samples in Fig. 2a. b, Fluorescence of TME with unfolded BLG in the presence of 0.025 – 8 mM GSH. Arrow indicates intracellular protein to glutathione thiol ratio, that is, 7:3. n = 4 biological replicates; mean ± s.d. c-d, TME fluorescence measured by the plate reader in Fig. 2d and e. Sample solutions were aliquoted from the in-gel fluorescence samples. n = 4 technical replicates; mean ± s.d. Results were consistent with kinetic curves (Fig. 2a and c).
