We present a cost-effective ultra-high-throughput cytometry-based framework for the detection of physical interactions between cells, along with the characterization of complex cellular landscapes. Application of our approach can offer a systems-level understanding of immunity and facilitate study of the kinetics, mode of action and personalized response prediction of immunotherapies.
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References
Nakandakari-Higa, S. et al. Universal recording of immune cell interactions in vivo. Nature 627, 399–406 (2024). This paper reports uLIPSTIC, a universal proximity-based labelling method to capture transient cell–cell interactions.
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This is a summary of: Vonficht, D. et al. Ultra-high-scale cytometry-based cellular interaction mapping. Nat. Methods https://doi.org/10.1038/s41592-025-02744-w (2025).
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Unravelling cellular interactions using flow cytometry. Nat Methods 22, 1770–1771 (2025). https://doi.org/10.1038/s41592-025-02743-x
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DOI: https://doi.org/10.1038/s41592-025-02743-x
