Extended Data Fig. 4: Multi-omic characterization of hAZFa in S. cerevisiae.
From: De novo assembly and delivery of synthetic megabase-scale human DNA into mouse early embryos
a, Genome browser view showing expression of hAZFa have transcripts in yeast. b, Proteomic enrichment analysis of Yeast_hAZFa compared with Yeast_Control, with circle sizes representing protein number and color intensity indicating significance level. c, Total RNA yields from Yeast_hAZFa were similar to those from Yeast_Control. n = 3 biological replicates. d, Whole-protein extracts of Yeast_Control and Yeast_hAZFa were run on a 12% SDS-Bis-Tris acrylamide gel and stained with Coomassie blue, using NEB #P7712 as the protein marker. n = 3 biological replicates. e, 3D structure model shows interactions of hAZFa and endogenous chromosomes in yeast. f, Z-score difference contact heatmap shows a comparison of chromosome interactions of Yeast_hAZFa and Yeast_Control. Bin length, 10 kb; red and blue show increased and decreased chromatin interactions, respectively. g, Comparison of the endogenous yeast centromere relative interaction frequency between Yeast_hAZFa and Yeast_control. Data are presented as the mean ± SE. Two-tailed t-tests were used to evaluate differences between groups. (***) P < 0.001.
