Extended Data Fig. 8: Transcriptional regulation of 76-kb BAC and hAZFa.
From: De novo assembly and delivery of synthetic megabase-scale human DNA into mouse early embryos
a, Genome browser view showing RNA-seq signals of the DDX3Y genes on hAZFa in mouse four-cell and morula embryos. b, Left, construction and microinjection of 76-kb BAC containing full-length TTTY15 and DDX3Y genes with flanking 5-kb regions. Right, RNA-seq of TTTY15 and DDX3Y genes on BAC in one-cell and early two-cell mouse embryos. c, Scatter plot showing gene expression fold-change upon adding 5-azadC, DMOG, and DMOG with 12 h postponing (2 biological replicates). FC, fold-change. Red lines, local regression fitting.
