Extended Data Fig. 9: De novo assembly of the 498-kb human AZFc region using SynNICE.
From: De novo assembly and delivery of synthetic megabase-scale human DNA into mouse early embryos
a, 498 kb human AZFc region schematic. b, Restriction digestion analysis of ten 50-kb DNA fragments followed by PFGE. NotI digestion and λ DNA-Mono Cut Mix (NEB, N3019S) as a marker. n = 3 biological replicates. c, Restriction digestion analysis of 249.4 kb DNA fragments C51-100 and C101-150 followed by PFGE. MluI digestion and λ PFG ladder (NEB, N0341S) as a marker. n = 2 biological replicates. d, Restriction digestion analysis of 498 kb DNA fragment C51-150 followed by PFGE. MluI digestion (left) and BsiWI digestion (right), λ PFG ladder as a marker. n = 2 biological replicates.
