Fig. 4: Chromatin remodeling of hAZFa in mouse early embryos.
From: De novo assembly and delivery of synthetic megabase-scale human DNA into mouse early embryos
a, Graphic illustration of hAZFa remodeling in mouse early embryos. b, Staining for the isolated yeast nucleus after injection into mouse MII oocytes with immediate fixation. Yeast nuclei membrane stained with DiIC12(3) (red) and DNA stained with DAPI (blue). The dashed circle represents the location of murine chromosome DNA. Scale bars, 20 µm. A magnified view shows the yeast nucleus in the oocyte cytoplasm. Scale bars, 1 µm. Quantification of three replicate experiments of yeast nuclei injected into mouse MII oocytes. Sample sizes are as follows: n = 11, 11 and 11. c, Live images of murine histones H3.3 (green) and H2B (red) incorporated into the yeast nucleus at 6 hours after injection. Scale bars, 20 µm. A magnified view shows the yeast nucleus in the cytoplasm. Scale bars, 1 µm. Quantification of three replicate experiments of murine histone incorporation. Sample sizes are as follows: n = 16, 21 and 11. d, Still images of timelapse imaging of H3.3–GFP in mouse MII oocytes. Timepoints represent hours after injection of yeast nuclei. Yeast nuclei with H3.3–GFP incorporation are indicated by yellow dashed circles. h, hours; TSS, transcription start site.
