Extended Data Fig. 6: Quantitative comparison with Slit Confocal Airy LS and standard Airy LS in different signal locations.

a,b, Representative images (a) and quantification of image contrast (b) for somata. c,d, Representative images (c) and quantification of image contrast (d) for neurites. All images were acquired with the same laser power to ensure equivalent maximum signal intensity. Data are presented as mean ± s.e.m. (n = 5 for both). Statistical significance was determined by a two-tailed, paired Student’s t-test (P = 0.0011 for somata; P = 0.0001 for neurites). On the graph, asterisks denote significance levels: **P < 0.01, ***P < 0.001.