Extended Data Fig. 2: Comparative analysis of Airy, deconvolved Airy, and confocal Airy microscopy. | Nature Methods

Extended Data Fig. 2: Comparative analysis of Airy, deconvolved Airy, and confocal Airy microscopy.

From: Confocal Airy beam oblique light-sheet tomography for brain-wide cell type distribution and morphology

Extended Data Fig. 2

a, Representative neurite images. b, Normalized intensity profiles of a. c, Quantitative comparison of image contrast across the three microscopy modes. Data are presented as mean ± s.e.m. (n = 5 neurites). Statistical analysis was performed using a one-way ANOVA with Tukey’s post-hoc test. Significant differences were found between the standard Airy (Control) and both Deconvolved (P = 0.0013) and Confocal (P = 0.0019) modes. No significant difference was observed between the Deconvolved and Confocal modes (P = 0.976). On the graph, asterisks denote significance levels: **P < 0.01.

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