Extended Data Fig. 4: Axial resolution and detection efficiency across the FOV. | Nature Methods

Extended Data Fig. 4: Axial resolution and detection efficiency across the FOV.

From: Confocal Airy beam oblique light-sheet tomography for brain-wide cell type distribution and morphology

Extended Data Fig. 4

(a) Maximum intensity projection of fluorescent microspheres across the full FOV. (b) Representative images of isolated microspheres at different Z’ positions (1: 250 μm, 2: 131 μm, 3: 491 μm). (c, e) Full-width at half maximum (FWHM) in X, Y, and Z for microspheres imaged at the original full FOV (c) and with Z-stitching (e). Original FOV (median ± s.d.): X: 0.78 ± 0.09 μm; Y: 0.46 ± 0.08 μm; Z: 2.95 ± 0.89 μm; Stitching FOV (median ± s.d.): X: 0.77 ± 0.10 μm; Y: 0.49 ± 0.08 μm; Z: 2.61 ± 0.79 μm. (d) Schematic of the Z-stitching strategy. (f) Background image from a median-filtered AVP brain. (g) Intensity profiles of microspheres versus distance from the FOV center (Z’). Data are presented as median± s.d.

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