Extended Data Fig. 4: Processing of β-galactosidase data for cryo-EM sample prepared starting from 1 μg βG containing cytoplasmic extract. | Nature Methods

Extended Data Fig. 4: Processing of β-galactosidase data for cryo-EM sample prepared starting from 1 μg βG containing cytoplasmic extract.

From: MISO: microfluidic protein isolation enables single-particle cryo-EM structure determination from a single cell colony

Extended Data Fig. 4

a, A micrograph; representative from n > 5000 micrographs. b, 2D class averages. The box size is 280 Å. c, Image processing scheme. d, Fourier Shell Correlation curves for unmasked and masked half-maps and between model and map. e, Heat map shows distribution of particle orientations. f, 3D map surface colored by local resolution.

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