Extended Data Fig. 7: Processing of TMEM16F-YFP purified and plunged using MISO. | Nature Methods

Extended Data Fig. 7: Processing of TMEM16F-YFP purified and plunged using MISO.

From: MISO: microfluidic protein isolation enables single-particle cryo-EM structure determination from a single cell colony

Extended Data Fig. 7

a, A micrograph; representative from n > 12000 micrographs. b, Selected 2D class averages. The box size is 278 Å. c, Image processing scheme. d, Fourier Shell Correlation curves for unmasked and masked half-maps and between model and map. e, Heat map shows distribution of particle orientations. f, 3D map surface colored by local resolution.

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