Extended Data Fig. 9: Identification of the TRPC6 by sequencing cryo-EM map. | Nature Methods

Extended Data Fig. 9: Identification of the TRPC6 by sequencing cryo-EM map.

From: MISO: microfluidic protein isolation enables single-particle cryo-EM structure determination from a single cell colony

Extended Data Fig. 9

a, ModelAngelo modelled a sequence of 151 HHM segments into a reconstructed cryo-EM map of the protein of interest. TRPC6 was identified as the highest likelihood target upon HHM profiles searched against the HMMER v3.3 reference proteome database. hTRPC6 was refined into the density (pink) and superimposed onto hTRPC6 (PDB 6UZ8, grey). b, Examples of density map regions with ModelAngelo sculptured fragments. c, Examples of HMMR search results for 2 sequences against the database.

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