Extended Data Fig. 7: Supporting data for the validation of cell type annotations. | Nature Methods

Extended Data Fig. 7: Supporting data for the validation of cell type annotations.

From: FX-Cell: a method for single-cell RNA sequencing on difficult-to-digest and cryopreserved plant samples

Extended Data Fig. 7: Supporting data for the validation of cell type annotations.

Sequential fluorescence in situ hybridization (seq-FISH) images showing expression patterns of selected cell cluster-specific genes in rice tiller nodes (a) and wild rice rhizome nodes (b). The images display transverse sections of the tiller nodes, rhizome nodes, and roots. The expression of each gene is represented on the left by UMAP and on the right by seq-FISH experiments. The gene name and the corresponding cell cluster are indicated at the top. Each gene’s transcripts are shown in different colors, while nuclei are stained with DAPI (4’,6-diamidino-2-phenylindole, grey). Scale bars, 50 μm. Two independent experiments were performed.

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