Extended Data Fig. 2: Supporting data for the generation of the maize anther cell atlas by the fixation and digestion at high temperatures protocol and CEL-seq2.
a, UMAP clustering of 307 cells from 2.0 mm maize anthers. Six distinct clusters are shown in different colors. Maize anthers were fixed and digested with RNAse-depleted enzymes at 50 °C for 90 min. The resultant cells were sorted and isolated using either BioSorter (Union Biometrica) or Hana (Namocell) machines into 96-well plates, and scRNA-seq libraries were prepared using a modified CEL-Seq2 library preparation protocol. b, Comparison of different cell isolation method (Biosorter vs. Hana). Each dot represents a single cell. c–e, Correlation values of each cell with LCM tapetal (c), meiocyte (d), and other somatic cell types (middle layer, endothecium, and epidermis) (e) data. f, Percentage of total UMIs originating from the plastid for each cell. Please note that Murphy et al. uncovered that the endothecium contains chloroplasts unlike the other anther cell layers74. g–j, UMAP plots showing expression patterns of tapetal (g), meiocyte (h), putative endothecium (i), and putative epidermis (j) marker genes. The corresponding cell clusters are labeled by dashed circles.
