Fig. 3: Longitudinal clonal tracking with TIRTL-seq.
From: TIRTL-seq: deep, quantitative and affordable paired TCR repertoire sequencing
a, Longitudinal sampling of a donor with SARS-CoV-2 infection. scGEX, single-cell gene expression. b, Top: TIRTL-seq identifies expansions and contractions in the CD8+ T cell repertoire. Bottom: CD4+ T cell clonal frequencies from the same time points. The dashed diagonal indicates the line of equality. c, Colored dots show clonotypes matching known TCRs specific for A*02 YLQ (cyan) and B*07 SPR SARS-CoV-2 epitopes (green) and A*02 GLC (orange) and B*07 RAK EBV epitopes (red) on pairwise time point comparisons. The dashed diagonal indicates the line of equality. d, Cumulative frequency of CD8+ clones specific to A*02 YLQ (cyan) and B*07 SPR SARS-CoV-2 epitopes (green) and A*02 GLC (orange) and B*07 RAK EBV epitopes (red) across time points. conv, convalescent. Panel a created with BioRender.com.
