Extended Data Fig. 8: Resolution analysis for 4Pi-SIMFLUX in mitochondria imaging.
From: 4Pi-SIMFLUX: 4Pi single-molecule localization microscopy with structured illumination
The mitochondria image shown in Fig. 5d was reconstructed from four optical sections. a, DAFL precision of molecules from each optical section. b, FRC resolution for each optical section. c, Distributions of 3D localization results from molecules emitting ≥10 frames. The photon count for these molecules is 4,478 ± 1,631 (mean ± s.d.). d, Histograms of the distributions in c were fit with Gaussian functions, and the standard deviations are reported.
