Extended Data Fig. 2: Optical layout of 4Pi-SIMFLUX.
From: 4Pi-SIMFLUX: 4Pi single-molecule localization microscopy with structured illumination
Refer to the Methods section for a detailed description. a, Structured illumination path. b, Fluorescence detection path. c, Focus-lock and salvaged fluorescence detection paths. AOTF: acousto-optic tunable filter; SMF: single-mode fiber; HWP: half-wave plate; SLM: spatial light modulator; FSW: four-segmented wave plate; DM1-4: dichroic mirror; QWP1-3: quarter-wave plate; Def. M1, 2: deformable mirror; NPBS: nonpolarizing beam splitter; AP1, 2: aperture; PBS: polarizing beam splitter; F1, 2: emission filter; L1-L16: lens; M1-M16: mirror; CY1: cylindrical lens; CAM1: sCMOS camera; CAM2: EMCCD camera; CAM3: CMOS camera.
