Extended Data Fig. 4: Synchronization diagram of 4Pi-SIMFLUX.
From: 4Pi-SIMFLUX: 4Pi single-molecule localization microscopy with structured illumination
The SLM served as the initial trigger signal, which was transmitted to the sCMOS camera with a 1 ms delay. The EMCCD was triggered once every six sCMOS exposures, and the AOTF remained open. One full 4Pi-SIMFLUX imaging cycle is 36 ms.
