Extended Data Fig. 5: Affinity of β-arrestin1 sensors towards V2Rpp measured using PRE-smFRET.
From: Parallel stopped-flow interrogation of diverse biological systems at the single-molecule scale
Apparent dissociation constant (\({\rm{K}}_{\rm{D}}^{\rm{app}}\)) estimated from steady-state V2Rpp titrations against β-arrestin1 sensors. (a) Comparison of \({\rm{K}}_{\rm{D}}^{\rm{app}}\) for sensors used for steady-state and pre-steady-state studies. (b) Effect of labelling-site mimicking mutations in C-tail 1 and C-domain rotation sensors. For both (a) and (b), plots show mean and SD of \({\rm{K}}_{\rm{D}}^{\rm{app}}\) values determined from 3 (C-tail 2, Finger loop, C-tail 1 D69A, C-tail1 L191A, C-domain rotation D69A, C-domain rotation K397A) or 9 (C-tail 1, C-domain rotation) independent repeats. P values are from independent two-tail t-tests.
