Extended Data Fig. 3: Microarrayed microfluidic devices for PRE-smFRET display high binding specificity for a range of biological samples.
From: Parallel stopped-flow interrogation of diverse biological systems at the single-molecule scale
(a) Sequential addition of bacterial initiation complexes containing unique tether domains demonstrates high localization accuracy. Quantification of binding specificity for: (b) FRET labelled double-stranded DNA, specificity of 98 ± 2% (c) FRET labelled β-arrestin1, specificity of 96 ± 2% (d) and bacterial initiation complexes containing Cy3-labelled fMet- tRNAfMet, specificity of 95 ± 2%. Samples prepared as described in Methods. Created with BioRender.com.
