Extended Data Fig. 6: Emission curves under single-photon excitation upon 450-nm illumination.
a–d, Purified protein (0.1 mg/mL) was used to analyze were used to analyze the emission spectra of mScarlet3 (a), mScarlet3-S2 (b), mScarlet3-M163H (c), and mScarlet-H (d) recorded with 450-nm excitation measured by the fluorescence spectrophotometer F7000. Protein purification was performed for three biologically independent replicates.
