Extended Data Fig. 8: mScarlet3-S2 is suitable for dual-color imaging when combined with mStayGold or mEmerald.
a. Fluorescence intensity distribution of various fluorescent proteins. U-2 OS cells transfected with mTurquoise2-P2A-mScarlet3-S2, mTurquoise2-P2A-mScarlet3, mTurquoise2-P2A-mSG, or mTurquoise2-P2A-mEmerald were collected, and fluorescence intensity of all cells in the green channel versus the blue channel by flow cytometry was plotted. No gating was used, and all cells were collected. k represents the slope of the linear regression. b. Dual-color imaging of mScarlet3-S2 with green fluorescent proteins. Live U-2OS cells were co-transfected with either: H2B-mScarlet3-S2 and Tom20-Emerald, or 2×MLS-mScarlet3-S2 and H2B-mStayGold. Images were acquired using Airyscan microscopy. Scale bar: 5 μm. Images were acquired on a Zeiss LSM 980 with Airyscan 2. The experiment was independently repeated three times with two technical replicates each, yielding similar results.
