Extended Data Fig. 9: Photochromic behavior of RFPs in U-2 OS cells.
a–g, The cells were WF illuminated with alternating light of 561 nm and 405 nm (a–c) or 488 nm (e–g) lights for multiple illumination cycles. Boxplot of all determined photochromic amplitudes (blue arrows) of RFPs determined with 5 illumination cycles are presented in (d) and (h). d, n = 15 cells for mScarlet3-S2, n = 10 cells for mScarlet3 and n = 15 cells for TagRFP-T. h, n = 15 cells for mScarlet3-S2, n = 5 cells for mScarlet3 and n = 15 cells for TagRFP-T. For the box-and-whisker plots, the center line represents the median, the box spans the interquartile range (IQR; 25th to 75th percentiles), and whiskers extend to 1.5× IQR. Outliers are plotted as individual points. Sample sizes (n) are indicated above each group.
