Extended Data Fig. 3: Monomeric properties of FPs.
a, Size-exclusion chromatography of mScarlet3-S2, mScarlet3-M163H, mScarlet3, mScarlet-H, StayGold and dTomato. b, The organized smooth endoplasmic reticulum (OSER) assay of variant FPs. Statistical analysis of the number of normal cells expressing mScarlet variant-labeled CytEMR is shown. c, Representative images of normal cells expressing mScarlet variant-labeled CytEMR, as well as abnormal cells expressing StayGold and dTomato. Scale bars, 10 μm. The experiment was independently repeated three times, with two technical replicates each, yielding similar results.
