Table 1 Characteristics of mScarlet3-S2

FPs	Ex^a (nm)	Em^b (nm)	EC^c (10³ M⁻¹ cm⁻¹)	QY^d	Brightness		pK_a^g	Photostability WF t_1/2 (s)			Maturation^k (min)
FPs	Ex^a (nm)	Em^b (nm)	EC^c (10³ M⁻¹ cm⁻¹)	QY^d	Molecular^e	In cells (%)^f	pK_a^g	1.9^h W cm⁻²	5.2ⁱ W cm⁻²	25^j W cm⁻²	Maturation^k (min)
mScarlet3-S2	550	587	83	0.18	15.0	20	3.3	1403	380	66.2	44
mScarlet3-M163H	552	588	104	0.15	15.6	22	3.4	886	187	28.8	44
mScarlet-H	552	589	71	0.19	13.5	18	4 6	706	146	23.4	138
mScarlet3	569	590	110	0.64	70.4	100	4 4	48	18	6.1	40

^aExcitation maximum. ^bEmission maximum. ^cExtinction coefficient at maximum absorbance. ^dQuantum yield. ^eCalculated molecular brightness, product of extinction coefficient and quantum yield. ^fBrightness in mammalian cells normalized to mScarlet3. ^gApparent pK_a value. ^h,i,jTime in seconds to half the emission rate under WF conditions at 1.9 W cm⁻², 5.2 W cm⁻² and 25 W cm⁻², respectively. ^kThe t_1/2 of chromophore maturation time.

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