Extended Data Fig. 1: Detection of barcodes with smFISH.
From: SpaceBar enables single-cell-resolution clone tracing with imaging-based spatial transcriptomics
Microscopy image depicting single-molecule FISH of melanoma cells transduced with a lentiviral pool containing 3 of the SpaceBar barcodes (barcodes 1, 2, and 3). DAPI is shown in gray. Probes targeting barcodes 1, 2, and 3 were coupled to Atto 674n (pseudocolored purple), Cy3 (pseudocolored orange), and Alexa Fluor 594 (pseudocolored green), respectively. Merge of all channels shown in A. Each barcode channel is shown separately in B-D.
