Extended Data Fig. 9: Effect of C21 is not different in cells from male and female animals. | Nature Neuroscience

Extended Data Fig. 9: Effect of C21 is not different in cells from male and female animals.

From: Sex-biased neural encoding of threat discrimination in nucleus accumbens afferents drives suppression of reward behavior

Extended Data Fig. 9

Bath application of C21 decreases membrane potential of (a) mPFC-NAc neurons expressing AAV-hM4Di in both male and female animals with no effect of sex (Ftreatment (1,9) = 33.17 n = 5,6 p = 0.0003; post hoc: pM = 0.0013, pF = 0.0061). (b) Representative traces illustrates effect of C21 (gray bar) in mPFC-NAc in males (upper trace) and females (lower trace). (c) The same effect was observed in vHip-NAc neurons expressing AAV-hM4Di in male and female animals again with no effect of sex (Ftreatment (1,11) = 36.12 n = 7,6 p < 0.0001; post-hoc: pM = 0.0007, pF = 0.0025). (d) Representative traces illustrates effect of C21 in vHip-NAc (gray bar) in males (upper trace) and females (lower trace). All tests are two-way RM Mixed effects analysis with Sidak’s multiple comparisons test. Data are presented as mean values +/− SEM. #p < 0.1, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Traces show representative current clamp recordings.

Source data

Back to article page