Extended Data Fig. 7: Localization of GluD2 and ADGRB3/BAI3 receptors in cerebellar Purkinje cells during postnatal development.
From: Stepwise molecular specification of excitatory synapse diversity onto cerebellar Purkinje cells
a,b, Immunostaining of GluD2 or ADGRB3/BAI3 receptors (green), CF presynaptic boutons (anti-VGLUT2, magenta) and PCs dendritic tree (anti-CaBP, blue) in parasagittal cerebellar sections from P4 and P13 mice. Left: low magnification images correspond to the projection of 5 planes (z-stack step: 0.2 µm). Scale bars, 20 µm. Right: airyscan images corresponding to single planes. Arrowheads show receptor labeling partially colocalizing with VGLUT2 clusters. Note that at P13, some GluD2 puncta are still detectable at CF/PC synapses. Three independent experiments. Scale bars, 4 µm.
