Extended Data Fig. 3: Micronuclei are incorporated into microglia.
From: Propagation of neuronal micronuclei regulates microglial characteristics
(a) Schematic illustration for an in vitro micronuclear transfer assay. (b) TEM image of BV2 cells after treatment with conditioned medium. NE: nuclear envelope, MN: micronucleus, Cyto: cytoplasm. (c) Immunostaining of BV2 cells with GFP−-and GFP+-MN. (d) Immunostaining of GFP−- and GFP+-MN in BV2. Yellow arrow indicates micronucleus. (e) The graph shows the percentage of neuronal MN (GFP+ MN) containing BV2 cells after treatment with a conditioned medium. Each spot indicates the combined value of 10 images from one experiment. n=3 independent experiments. NB; fresh neurobasal medium, mean ± SEM, p values were analyzed by one-way ANOVA Dunnett's multiple comparisons test. (f) Schematic illustration for eliminating MN. (g) The graph shows MN+ BV2 cells 6 hours after treatment with a conditioned medium. mean ± SEM, p value was calculated by one-way ANOVA Tukey's multiple comparisons tests. Each spot indicates the combined value of more than 9 images from one experiment. Each column indicates a set of 3 independent experiments. (h) Live imaging of Cx3cr1-EGFP;H2B-mCherry cortical slice culture (P3). The interval of taking images was every 5 min. Yellow arrowheads indicate MN in the microglia. Cyan asterisks indicate MN out of the microglia. No.1-6, 89 z-stack images/field, No. 7-24, 92 z-stack images/field, 1.5 µm pitch.
