Extended Data Fig. 1: Immunological Characterization of CIR.

Upper box: Gating strategy. Peritoneal cells were gated on a forward scatter (FSC)/side scatter (SSC) plot (I) and FSC-W FSC-H to identify single cells (II). (III) Lymphocytes were gated to determine macrophages and monocyte populations. F4/80 + Ly6C- cells we re-gated to determine CD80 (IV) and CD86 (V) positive cells. Blue Box: ILs concentration in the peritoneal lavage. a)[IL1b]:LPS-water (0 ± 0 pg/ml), vehicle (0 ± 0 pg/ml), LPS-LPS (0 ± 0 pg/ml), CIR (0.3500 ± 0.2391 pg/ml) and water (0 ± 0 pg/ml) Kruskal-Wallis test, p = 0.1072. b) [IL-2]: LPS-water (0 ± 0 pg/ml), vehicle (0.1167 ± 0.0792 pg/ml), LPS-LPS (0 ± 0 pg/ml), CIR (0 ± 0 pg/ml) and water (0 ± 0 pg/ml) Kruskal-Wallis test, p = 0.0285. c) [IL-4]: LPS-water (0 ± 0 pg/ml), vehicle (0 ± 0 pg/ml), LPS-LPS (0 ± 0 pg/ml), CIR (0 ± 0 pg/ml) and water (0 ± 0 pg/ml). d) [IL-5]: LPS-water (0 ± 0 pg/ml), vehicle (0 ± 0 pg/ml), LPS-LPS (0 ± 0 pg/ml), CIR (0 ± 0 pg/ml) and water (0 ± 0 pg/ml). e) [IL-6]:LPS-LPS (6.120 ± 1.968 pg/ml) higher than LPS-water (0 ± 0 pg/ml, p < 0.0001), vehicle (0 ± 0 pg/ml, p < 0.0001), CIR (0 ± 0 pg/ml, p < 0.0001) and water (0 ± 0 pg/ml, p = 0.0003) Kruskal-Wallis test, p < 0.0001. f) [IL-10]: LPS-water (0 ± 0 pg/ml), vehicle (0 ± 0 pg/ml), LPS-LPS (0 ± 0 pg/ml), CIR (0 ± 0 pg/ml) and water (0 ± 0 pg/ml). g) [IL-12]: LPS-water (0 ± 0 pg/ml; p < 0.0001), vehicle (0 ± 0 pg/ml), LPS-LPS (0 ± 0 pg/ml), CIR (0 ± 0 pg/ml) and water (0 ± 0 pg/ml). h) [IL-13] higher in water (34.84 ± 11.24 pg/ml) than vehicle (3.117 ± 1.473 pg/ml, Dunn’s multiple comparisons test, p = 0.0107). CIR (9.950 ± 3.298 pg/ml), LPS-water (21.13 ± 9.019 pg/ml) and LPS-LPS (7.980 ± 2.038 pg/ml) (Kruskal-Wallis test, p = 0.0242) i) [IL-17]: LPS-water (0 ± 0 pg/ml), vehicle (0 ± 0 pg/ml), LPS-LPS (0 ± 0 pg/ml), CIR (0 ± 0 pg/ml) and water (0 ± 0 pg/ml) j) [IL-17F]: LPS-water (0 ± 0 pg/ml), vehicle (0 ± 0 pg/ml), LPS-LPS (0 ± 0 pg/ml), CIR (0 ± 0 pg/ml) and water (0 ± 0 pg/ml). k) [IL-21] LPS-water (0 ± 0 pg/ml), vehicle (0 ± 0 pg/ml), LPS-LPS (0 ± 0 pg/ml), CIR (0 ± 0 pg/ml) and water (0 ± 0 pg/ml). l) [IL-22]: LPS-water (0 ± 0 pg/ml), vehicle (0 ± 0 pg/ml), LPS-LPS (0 ± 0 pg/ml), CIR (0 ± 0 pg/ml) and water (0 ± 0 pg/ml). m) [IL-28]: LPS-water (0 ± 0 pg/ml), vehicle (0 ± 0 pg/ml), LPS-LPS (0 ± 0 pg/ml), CIR (0 ± 0 pg/ml) and water (0 ± 0 pg/ml). n) [IFNG]: LPS-water (0 ± 0 pg/ml), vehicle (0 ± 0 pg/ml), LPS-LPS (0 ± 0 pg/ml), CIR (0 ± 0 pg/ml) and water (0 ± 0 pg/ml). o) [MIP3a] for LPS-LPS (5.700 ± 1.834 pg/ml) higher than LPS-water (0 ± 0 pg/ml, p = 0.0008), vehicle (0 ± 0 pg/ml, p = 0.0008), CIR (0 ± 0 pg/ml, p = 0.0014) and water (0 ± 0 pg/ml, p = 0.0026) Kruskal-Wallis test, p = 0.0002. p) [TGFb1]: LPS-water (238.7 ± 197.9 pg/ml), vehicle (1476 ± 832.9 pg/ml), LPS-LPS (0 ± 0 pg/ml), CIR (0 ± 0 pg/ml) and water (0 ± 0 pg/ml) (Kruskal-Wallis test, p = 0.0389). q) [TNFa]: LPS-water (14.57 ± 4.932 pg/ml), vehicle (13.67 ± 4.067 pg/ml), LPS-LPS (7.440 ± 2.613 pg/ml), CIR (15.3 ± 5.192 pg/ml) and water (0 ± 0 pg/ml) Kruskal-Wallis test, p = 0.1661. Red Box: ILs concentration in the blood. A) [IL-1b]: LPS-water (0 ± 0 pg/ml), vehicle (0.8800 ± 0.6591 pg/ml), LPS-LPS (0 ± 0 pg/ml), CIR (0 ± 0 pg/ml), water (0 ± 0 pg/ml) Kruskal-Wallis test, p = 0.0801. B) [IL-2]: LPS-water (0.5 ± 0.3141 pg/ml), vehicle (1.133 ± 0.500 pg/ml), LPS-LPS (1.22 ± 05877 pg/ml), CIR (0.18 ± 0.12 pg/ml), water (0.12 ± 0.08 pg/ml) Kruskal-Wallis test, p = 0.4879. C), [IL-4]: LPS-water (0.18 ± 0.18 pg/ml), vehicle (6.050 ± 2.962 pg/ml), LPS-LPS (0 ± 0 pg/ml), CIR (3.817 ± 2.252 pg/ml), water (0 ± 0 pg/ml) Kruskal-Wallis test, p = 0.0386. D) [IL-5]: CIR (38.20 ± 11.08 pg/ml), water (13.66 ± 9.609 pg/ml), LPS-water (26.78 ± 12.28 pg/ml), vehicle (45.80 ± 24.51 pg/ml), LPS-LPS (42.64 ± 20.51 pg/ml) Kruskal-Wallis test, p = 0.0800. E) [IL-6] for LPS-LPS (2568 ± 1240 pg/ml) higher than LPS-water (0.0333 ± 0.02108 pg/ml, Dunn’s multiple comparisons, p = 0.0184), vehicle (0.0500 ± 0.03416 pg/ml, Dunn’s multiple comparisons, p = 0.0237), CIR (0.1667 ± 0.1085 pg/ml, Dunn’s multiple comparisons, p = 0.0438) and water (0 ± 0 pg/ml, Dunn’s multiple comparisons, p = 0.0021) Kruskal-Wallis test, p = 0.0024. F) [IL-10]; LPS-water (0 ± 0 pg/ml), vehicle (0 ± 0 pg/ml), LPS-LPS (0 ± 0 pg/ml), CIR (0 ± 0 pg/ml), water (0 ± 0 pg/ml). G) [IL-12]: LPS-water (0 ± 0 pg/ml), vehicle (0 ± 0 pg/ml), LPS-LPS (0 ± 0 pg/ml), CIR (0 ± 0 pg/ml), water (0 ± 0 pg/ml).H) [IL-13]: LPS-water (15.47 ± 5.689 pg/ml), vehicle (14.40 ± 4.231 pg/ml), LPS-LPS (7.575 ± 1.227 pg/ml), CIR (16.17 ± 7.980 pg/ml), water (20.80 ± 8.240 pg/ml) Kruskal-Wallis test, p = 0.6901. I) [IL-17} lower in LPS-LPS (0.800 ± 0.4243 pg/ml) than CIR (0 ± 0, Dunn’s multiple comparisons test, p = 0.0496) and vehicle (0 ± 0 pg/ml, Dunn’s multiple comparisons test, p = 0.0496) groups, but not from LPS-Water (0.350 ± 0.180 pg/ml) and water (0.05 ± 0.05 pg/ml) groups (Kruskal-Wallis test, p = 0.0164). J) [IL-17F]: LPS-water (0 ± 0 pg/ml), vehicle (0 ± 0 pg/ml), LPS-LPS (0 ± 0 pg/ml), CIR (0 ± 0 pg/ml), water (0 ± 0 pg/ml). K) [IL-21]: LPS-water (0 ± 0 pg/ml), vehicle (0 ± 0 pg/ml), LPS-LPS (0 ± 0 pg/ml), CIR (0 ± 0 pg/ml) and water (0 ± 0 pg/ml). L) [IL-22]: LPS-water (0 ± 0 pg/ml), vehicle (0 ± 0 pg/ml), LPS-LPS (0 ± 0 pg/ml), CIR (0 ± 0 pg/ml), water (0 ± 0 pg/ml). M)[IL-28]: LPS-water (0 ± 0 pg/ml), vehicle (0 ± 0 pg/ml), LPS-LPS (0 ± 0 pg/ml), CIR (0 ± 0 pg/ml) and water (0 ± 0 pg/ml). N)[IFNG]: CIR (83.68 ± 39.13 pg/ml), vehicle (88.97 ± 38.92 pg/ml), LPS-water (0 ± 0 pg/ml), LPS-LPS (0 ± 0 pg/ml), water (0 ± 0 pg/ml) Kruskal-Wallis test, p = 0.0036. O) [MIP3a] for LPS-LPS (2561 ± 688.5 pg/ml) higher than LPS-water (17.32 ± 3,934 pg/ml, Dunn’s multiple comparisons test, p = 0.0206) and vehicle (8.320 ± 1.670 pg/ml, Dunn’s multiple comparisons test, p = 0.0009) groups, and but not significantly different than CIR (60.27 ± 13.95 pg/ml) and water (101.7 ± 71.25 pg/ml) (Kruskal-Wallis test, p = 0.0012). P) [TGFb1]: LPS-water (957.1 ± 469.5 pg/ml), vehicle (1208 ± 1057 pg/ml), LPS-LPS (2939 ± 1895 pg/ml), CIR (1664 ± 872.4 pg/ml), water (151.8 ± 88.02 pg/ml) Kruskal-Wallis test, p = 0.8556.Q)[TNFa] for vehicle (45.52 ± 16.50 pg/ml) higher than water (2.420 ± 1.501 pg/ml, Dunn’s multiple comparisons test, p = 0.0101), but not significantly different than LPS-LPS (9.00 ± 5.789 pg/ml), LPS-water (7.917 ± 3.935 pg/ml), CIR (8.250 ± 3.181 pg/ml) Kruskal-Wallis test, p = 0.0113. Data are presented as means ± SEM (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).

Source data