Extended Data Fig. 3: CIR retrieval changes intrinsic properties of aIC-pIC projecting neurons.
Intrinsic properties for the aIC-to-pIC neurons (a-l): a, CIR (8.929 ± 1.413 mV, n = 15) showed an increased trend in fast after polarization potentials compared to the vehicle (5.618 ± 1.347 mV, n = 13) group and baseline (5.103 ± 1.347 mV,n = 12) but not significantly different, One-Way ANOVA Kruskal-Wallis test p = 0.1453. N = 8 mice. b, Medium after hyper polarization potentials were similar in the CIR group (-2.905 ± 0.649 mV, n = 15) compared to baseline (-3.376 ± 0.7747 mV, n = 12) and vehicle (-4.714 ± 0.8833 mV, n = 13; One-Way ANOVA, p = 0.2297). c, Slow after polarization potentials were not significantly different in between the CIR (-1.981 ± 0.3811 mV, n = 15), baseline (-1.258 ± 0.5244 mV, n = 12) and vehicle (-2.663 ± 0.755 mV, n = 13; One-Way ANOVA, p = 0.2448). d, The representative traces showing a broader action potential (half-width) in CIR group compared to the control groups (Scale bar 20 mV and 1 ms). e, Both vehicle (49.77 ± 5.435 mV, n = 13) and CIR (51.27 ± 3.183 mV, n = 15) groups showed a trend in decreased action potential amplitude compared to the baseline group (61.75 ± 3.881 mV, n = 12 but not significantly different, One-Way ANOVA, p = 0.1063). f, Action potential half-width is significantly enhanced in CIR group (1.564 ± 0.1862 ms, n = 15) compared to the baseline level (0.8233 ± 0.1083 ms, n = 12) but not significantly different than the vehicle group (1.160 ± 0.1580 ms, n = 13; One-Way ANOVA,Kruskal-Wallis test, p = 0.0082). g, Action potential threshold were not significantly different between the baseline (-33.31 ± 2.520 mV, n = 12), vehicle (-31.22 ± 1.604, mV n = 13), and CIR (-32.78 ± 1.258 mV, n = 15) groups (One-Way ANOVA, Kruskal-Wallis test, p = 0.5168). h, Resting membrane potentials were not significantly different between the baseline (-72.63 ± 1.868 mV, n = 12), vehicle (-71.25 ± 1.524 mV, n = 13), and CIR (-73.52 ± 1.013 mV, n = 15) groups (One-Way ANOVA, p = 0.5318). i, Rheobase were not significantly different between the baseline (61.25 ± 9.375 pA, n = 12), vehicle (94.23 ± 13.14 pA, n = 13), and CIR (75.87 ± 11.78 pA, n = 15) groups (One-Way ANOVA,Kruskal-Wallis test, p = 0.0935). j, Input resistance were not significantly different between the baseline (181.6 ± 16.60 MΩ, n = 12), vehicle (162.5 ± 15.45 MΩ, n = 13), and CIR (172.5 ± 13.08 MΩ, n = 15) groups (One-Way ANOVA, p = 0.6809). k, Sag ratio were similar in between the baseline (8.497 ± 2.089, n = 12), vehicle (9.182 ± 1.698, n = 13), and CIR (9.260 ± 1.282, n = 15) groups (One-Way ANOVA, p = 0.9403). l, The membrane time constants were significantly enhanced in CIR group (26.79 ± 2.008 ms, n = 15) compared to vehicle (21.93 ± 2.407 ms, n = 13) group, but was not significantly different than the baseline (21.12 ± 2.407 ms, n = 12) group (One-Way ANOVA, p = 0.0402). Figure was created using BioRender. Intrinsic properties for the pIC-to-aIC neurons (m-x): m, CIR retrieval showed a trend in an increased fast after polarization potentials (8.394 ± 1.841 mV, n = 10) compared to baseline (6.042 ± 1.55 mV, n = 11) and to vehicle (7.46 ± 1.442 mV, n = 13) group, but not significantly different (One-Way ANOVA, p = 0.6026). N = 8 mice. n, Medium hyperpolarization potentials were similar in the CIR group (-3.630 ± 1.022 mV, n = 10) compared to baseline (-5.625 ± 1.114 mV, n = 11) and to vehicle (-5363 ± 0.8790 mV, n = 13) groups (One-Way ANOVA, Kruskal-Wallis test, p = 0.2397). o, Slow after polarization potentials were not significantly different in between baseline (-2.674 ± 0.6305 mV, n = 11), vehicle (-2.863 ± 0.3580 mV, n = 13), and CIR (-2.123 ± 0.4064 mV, n = 10) groups (One-Way ANOVA, p = 0.5360). p, The representative traces showing a broader activation potential half-width in the CIR group compared to baseline and vehicle groups. Scale bar 20 mV and 1 ms. q, Both vehicle (44.58 ± 4.601 mV, n = 13) and CIR (51.28 ± 2.706 mV, n = 10) groups showed a similar action potential amplitude compared to the baseline group (55.94 ± 5.848 mV, n = 11; One-Way ANOVA, p = 0.2235). r, Action potential half-width is significantly enhanced in CIR group (1.322 ± 0.1499 ms, n = 10) compared to the baseline (0.7864 ± 0.07533 ms, n = 11), but was not significantly different than the vehicle (1.069 ± 0.09027 ms, n = 13) group (One-Way ANOVA,Kruskal-Wallis test, p = 0.0081). s, Action potential threshold were not significantly different between the baseline (-34.10 ± 1.991 mV, n = 11), vehicle (-35.28 ± 12.696, mV n = 13), and CIR (-30.17 ± 2.516 mV, n = 10) groups (One-Way ANOVA, p = 0.3374). t, Resting membrane potentials were not significantly different between the baseline (-71.91 ± 1.560 mV, n = 11), vehicle (-69.80 ± 1.537 mV, n = 13), and CIR (-74.46 ± 1.198 mV, n = 10) groups (One-Way ANOVA, p = 0.0994). u, Rheobase was not significantly different between the baseline (53.64 ± 6.372 pA, n = 11), vehicle (98.00 ± 19.06 pA, n = 13), and CIR (92.10 ± 12.83 pA, n = 10) groups (One-Way ANOVA, p = 0.0835). v, Input resistance was not significantly different between the baseline (188.1 ± 25.87 MΩ, n = 11), vehicle (156.0 ± 24.01MΩ, n = 13), and CIR (160.4 ± 11.36 MΩ, n = 13) groups (One-Way ANOVA, p = 0.5493). w, Sag ratio was similar in between the baseline (12.25 ± 2.637, n = 11), vehicle (10.68 ± 2.328, n = 13), and CIR (11.39 ± 1.682, n = 10) groups (One-Way ANOVA, p = 0.8859). x, The membrane time constants were significantly enhanced in CIR group (26.23 ± 2.872 ms, n = 10) compared to vehicle group (16.95 ± 1.915 ms, n = 13), and the baseline (15.48 ± 1.437 ms, n = 11; One-Way ANOVA, p = 0.0027). Values are expressed as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Figure was created using BioRender.
