Extended Data Fig. 1: Viral strategies for sparsely labeling hypothalamic peptidergic neurons.
From: Projectome-based characterization of hypothalamic peptidergic neurons in male mice
a. To sparsely label neuropeptide-expressing populations listed at the bottom, the indicated virus or virus mixture was injected into wild-type mice to label Orexin-expressing neurons or into Cre-expressing mice to label all other peptidergic populations. b. Representative images showing co-localization of EYFP or GFP signal with in situ or immunohistochemistry signals of the indicated neuropeptide (red). The quantification shows the percentage (%) of EYFP or GFP-labeled cells that co-expressed the neuropeptide. Data are presented as mean ± S.E.M. N indicates the number of biological replicates. The images on the right show magnified regions within the white box on the left. Scale bar, left, 100 μm; right, 20 μm.
