Extended Data Fig. 2: AI-guided myelin quantification in Cnp-EGFP-5XFAD and Cnp-EGFP-WT mice. | Nature Neuroscience

Extended Data Fig. 2: AI-guided myelin quantification in Cnp-EGFP-5XFAD and Cnp-EGFP-WT mice.

From: Myelin–axon interface vulnerability in Alzheimer’s disease revealed by subcellular proteomics and imaging of human and mouse brain

Extended Data Fig. 2

a. Myelin in Cnp-EGFP-5XFAD mice and Cnp-EGFP-WT mice was quantified using AI-guided segmentation of immunofluorescence confocal images. b. Representative images showing tiled confocal images of brains from Cnp-EGFP-WT and Cnp-EGFP-5XFAD mice. Myelin (grey) was labeled by anti-GFP, and lysosomes/dystrophic neurites (red) were labeled by anti-Lamp1. Brain regions used for quantification are marked by yellow boxes. Low zoom and high zoom representative images with myelin (anti-GFP), AI-generated masks and objects. Scale bars: full-tiled images = 500 μm; low zoom myelin images = 100 μm; high zoom images = 5 μm. c. Quantification of myelin volume and mean fluorescence intensity in regions of interest for Cnp-EGFP-5XFAD mice (n = 4) and Cnp-EGFP-WT mice (n = 5); (Mann Whitney test, two-tailed). Abbreviations: RSPd = Retrosplenial area, dorsal part; PTLp = Posterior parietal association areas; DG = dentate gyrus. Error bars indicate SEM.

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